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Radiation damage to GH7 Family Cellobiohydrolase from Daphnia pulex: Dose (DWD) 20.6 MGy

X-ray diffraction
2Å resolution
PDB entry 5mcm coloured by chain, front view.
PDB entry 5mcm coloured by chain, side view.
PDB entry 5mcm coloured by chain, top view.
Source organism: Daphnia pulex
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1
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Assembly Name: cellulose 1,4-beta-cellobiosidase (non-reducing end)
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Multimeric state: monomeric
Accessible surface area: 16750.56 Å2
Buried surface area: 294.93 Å2
Dissociation area: 147.47 Å2
Dissociation energy (ΔGdiss): 3.15 kcal/mol
Dissociation entropy (TΔSdiss): 0.86 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-123167
Assembly 2 (preferred)
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Assembly Name: cellulose 1,4-beta-cellobiosidase (non-reducing end)
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Multimeric state: monomeric
Accessible surface area: 16714.56 Å2
Buried surface area: 678.16 Å2
Dissociation area: 110.97 Å2
Dissociation energy (ΔGdiss): -0.2 kcal/mol
Dissociation entropy (TΔSdiss): 0.9 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-123167

Macromolecules

cellulose 1,4-beta-cellobiosidase (non-reducing end)
Chains: A, B
Length: 445 amino acids
Theoretical weight: 48.23 KDa
Source organism: Daphnia pulex
Expression system: Trichoderma reesei QM6a
UniProt:
  • Canonical: E9G5J5 (Residues: 20-464; Coverage: 100%)
Gene names: CEL7A, DAPPUDRAFT_347598
Pfam: Glycosyl hydrolase family 7
InterPro:
CATH: Glycoside hydrolase, family 7, domain
cellulose 1,4-beta-cellobiosidase (non-reducing end) in PDB entry 5mcm, assembly 2, front view.
cellulose 1,4-beta-cellobiosidase (non-reducing end) in PDB entry 5mcm, assembly 2, side view.
cellulose 1,4-beta-cellobiosidase (non-reducing end) in PDB entry 5mcm, assembly 2, top view.
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