5mcm

X-ray diffraction
2Å resolution

Radiation damage to GH7 Family Cellobiohydrolase from Daphnia pulex: Dose (DWD) 20.6 MGy

Released:
Source organism: Daphnia pulex
Primary publication:
OH cleavage from tyrosine: debunking a myth.
OpenAccess logo J Synchrotron Radiat 24 7-18 (2017)
PMID: 28009542

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cellobiohydrolase CHBI Chains: A, B
Molecule details ›
Chains: A, B
Length: 445 amino acids
Theoretical weight: 48.23 KDa
Source organism: Daphnia pulex
Expression system: Trichoderma reesei QM6a
UniProt:
  • Canonical: E9G5J5 (Residues: 20-464; Coverage: 100%)
Gene names: CEL7A, DAPPUDRAFT_347598
Sequence domains: Glycosyl hydrolase family 7
Structure domains: Glycoside hydrolase, family 7, domain

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: C2
Unit cell:
a: 127.471Å b: 46.77Å c: 174.08Å
α: 90° β: 108.2° γ: 90°
R-values:
R R work R free
0.232 0.232 0.252
Expression system: Trichoderma reesei QM6a