X-ray diffraction
2.78Å resolution

Human porphobilinogen deaminase in complex with DPM cofactor

Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
4 porphobilinogen + H(2)O = hydroxymethylbilane + 4 NH(3)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Porphobilinogen deaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 365 amino acids
Theoretical weight: 40.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P08397 (Residues: 18-361; Coverage: 95%)
Gene names: HMBS, PBGD, UPS
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand DPM 2 x DPM
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 70.42Å b: 80.95Å c: 76.22Å
α: 90° β: 95.7° γ: 90°
R R work R free
0.222 0.22 0.26
Expression system: Escherichia coli