› 5m56

X-ray diffraction
2.24Å resolution

Monoclinic complex structure of human protein kinase CK2 catalytic subunit (isoform CK2alpha') with the inhibitor 4'-carboxy-6,8-chloro-flavonol (FLC21)

Released:
DOI: 10.2210/pdb5m56/pdb
PDB 5m56 coloured by chain and viewed from the front.
PDB 5m56 coloured by chain and viewed from the side.
PDB 5m56 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural Hypervariability of the Two Human Protein Kinase CK2 Catalytic Subunit Paralogs Revealed by Complex Structures with a Flavonol- and a Thieno[2,3-d]pyrimidine-Based Inhibitor.
Niefind K, Bischoff N, Golub AG, Bdzhola VG, Balanda AO, Prykhod'ko AO, Yarmoluk SM
Pharmaceuticals (Basel) 10 (2017)
PMID: 28085026

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase II subunit alpha' Chains: A, B
Molecule details ›
Chains: A, B
Length: 364 amino acids
Theoretical weight: 42.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19784 (Residues: 1-350; Coverage: 100%)
Gene names: CK2A2, CSNK2A2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand 7FC 2 x 7FC
Ligand GOL 4 x GOL
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 69.341Å b: 87.62Å c: 72.977Å
α: 90° β: 109.69° γ: 90°
R-values:
R R work R free
0.165 0.164 0.206
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Conformational dynamics of human protein kinase CK2α and its effect on function and inhibition.
Srivastava et al. (2018)
3 more

3 mentions without citation

Structural Hypervariability of the Two Human Protein Kinase CK2 Catalytic Subunit Paralogs Revealed by Complex Structures with a Flavonol- and a Thieno[2,3-d]pyrimidine-Based Inhibitor.
Niefind et al. (2017)
2 more