5m4b

X-ray diffraction
1.5Å resolution

Alpha-amino epsilon-caprolactam racemase D210A mutant in complex with PLP and geminal diamine intermediate

Released:

Function and Biology Details

Reaction catalysed:
(S)-2-aminohexano-6-lactam = (R)-2-aminohexano-6-lactam
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class-III Chain: A
Molecule details ›
Chain: A
Length: 440 amino acids
Theoretical weight: 46 KDa
Source organism: Rhizobacter
Expression system: Escherichia coli
UniProt:
  • Canonical: N6UXY4 (Residues: 1-436; Coverage: 100%)
Gene name: RHSP_08934
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: C2
Unit cell:
a: 86.21Å b: 79.111Å c: 58.971Å
α: 90° β: 112.82° γ: 90°
R-values:
R R work R free
0.141 0.14 0.168
Expression system: Escherichia coli