5m3k

X-ray diffraction
2.83Å resolution

A multi-component acyltransferase PhlABC from Pseudomonas protegens

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
PhlA Chains: A, D
Molecule details ›
Chains: A, D
Length: 362 amino acids
Theoretical weight: 38.57 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9TP23 (Residues: 1-362; Coverage: 100%)
Gene names: PFL02_36210, phlA
Sequence domains: 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
2,4-diacetylphloroglucinol biosynthesis protein PhlB Chains: B, E
Molecule details ›
Chains: B, E
Length: 146 amino acids
Theoretical weight: 16.67 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4K419 (Residues: 1-146; Coverage: 100%)
Gene names: PFL_5956, phlB
Sequence domains:
Thiolase_N domain-containing protein Chains: C, F
Molecule details ›
Chains: C, F
Length: 398 amino acids
Theoretical weight: 42.58 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4K420 (Residues: 1-398; Coverage: 100%)
Gene names: PFL_5955, phlC
Sequence domains: Thiolase, N-terminal domain

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6122
Unit cell:
a: 222.626Å b: 222.626Å c: 237.099Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.167 0.196
Expression system: Escherichia coli