X-ray diffraction
2.8Å resolution

Structure of the hybrid domain (GGDEF-EAL) of PA0575 from Pseudomonas aeruginosa PAO1 at 2.8 Ang. with GTP and Ca2+ bound to the active site of the GGDEF domain


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Bifunctional diguanylate cyclase/phosphodiesterase Chains: A, B
Molecule details ›
Chains: A, B
Length: 440 amino acids
Theoretical weight: 49.08 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9I5W1 (Residues: 809-1245; Coverage: 36%)
Gene name: PA0575
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.3
Spacegroup: P21212
Unit cell:
a: 122.307Å b: 133.931Å c: 69.141Å
α: 90° β: 90° γ: 90°
R R work R free
0.245 0.244 0.277
Expression system: Escherichia coli BL21(DE3)