› 5m34

X-ray diffraction
1.6Å resolution

Structure of cobinamide-bound BtuF mutant W66Y, the periplasmic vitamin B12 binding protein in E.coli

Released:
DOI: 10.2210/pdb5m34/pdb
PDB entry 5m34 coloured by chain, front view.
PDB entry 5m34 coloured by chain, side view.
PDB entry 5m34 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Conformational Change of a Tryptophan Residue in BtuF Facilitates Binding and Transport of Cobinamide by the Vitamin B12 Transporter BtuCD-F.
Mireku SA, Ruetz M, Zhou T, Korkhov VM, Kräutler B, Locher KP
Sci Rep 7 41575 (2017)
PMID: 28128319

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153331 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vitamin B12-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 31.49 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P37028 (Residues: 22-266; Coverage: 100%)
Gene names: JW0154, b0158, btuF, yadT
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

3 bound ligands:
Ligand CBY 2 x CBY
Ligand CYN 3 x CYN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 139.83Å b: 90.8Å c: 51.07Å
α: 90° β: 111.25° γ: 90°
R-values:
R R work R free
0.193 0.191 0.233
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Sharing vitamins: Cobamides unveil microbial interactions.
Sokolovskaya et al. (2020)
8 other articles

1 mention without citation

Conformational Change of a Tryptophan Residue in BtuF Facilitates Binding and Transport of Cobinamide by the Vitamin B12 Transporter BtuCD-F.
Mireku et al. (2017)