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The Toxin-Antitoxin System DarTG Catalyzes Reversible ADP-Ribosylation of DNA.

Jankevicius G, Ariza A, Ahel M, Ahel I
Mol Cell 64 1109-1116 (2016)
Related entries: 5m3e, 5m3i

Cited: 88 times
EuropePMC logo PMID: 27939941

Abstract

The discovery and study of toxin-antitoxin (TA) systems helps us advance our understanding of the strategies prokaryotes employ to regulate cellular processes related to the general stress response, such as defense against phages, growth control, biofilm formation, persistence, and programmed cell death. Here we identify and characterize a TA system found in various bacteria, including the global pathogen Mycobacterium tuberculosis. The toxin of the system (DarT) is a domain of unknown function (DUF) 4433, and the antitoxin (DarG) a macrodomain protein. We demonstrate that DarT is an enzyme that specifically modifies thymidines on single-stranded DNA in a sequence-specific manner by a nucleotide-type modification called ADP-ribosylation. We also show that this modification can be removed by DarG. Our results provide an example of reversible DNA ADP-ribosylation, and we anticipate potential therapeutic benefits by targeting this enzyme-enzyme TA system in bacterial pathogens such as M. tuberculosis.

Reviews - 5m31 mentioned but not cited (1)

  1. ADP-ribosylation systems in bacteria and viruses. Mikolčević P, Hloušek-Kasun A, Ahel I, Mikoč A. Comput Struct Biotechnol J 19 2366-2383 (2021)


Reviews citing this publication (37)

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Articles citing this publication (50)

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