PDBe 5m2s

X-ray diffraction
1.7Å resolution

R. flavefaciens' third ScaB cohesin in complex with a group 1 dockerin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Putative cellulosomal scaffoldin protein Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 15.93 KDa
Source organism: Ruminococcus flavefaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0AEF3 (Residues: 465-610; Coverage: 21%)
Gene name: scaA
Doc8: Type I dockerin repeat domain from family 9 glycoside hydrolase WP_009982745[Ruminococcus flavefaciens] Chain: B
Molecule details ›
Chain: B
Length: 103 amino acids
Theoretical weight: 10.97 KDa
Source organism: Ruminococcus flavefaciens FD-1
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 45.61Å b: 64.49Å c: 47.67Å
α: 90° β: 116.72° γ: 90°
R-values:
R R work R free
0.135 0.134 0.157
Expression system: Escherichia coli BL21(DE3)