› 5m1j

Electron Microscopy
3.3Å resolution

Nonstop ribosomal complex bound with Dom34 and Hbs1

Released:
DOI: 10.2210/pdb5m1j/pdb
PDB 5m1j coloured by chain and viewed from the front.
PDB 5m1j coloured by chain and viewed from the side.
PDB 5m1j coloured by chain and viewed from the top.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution.
Hilal T, Yamamoto H, Loerke J, Bürger J, Mielke T, Spahn CM
OpenAccess logo Nat Commun 7 13521 (2016)
PMID: 27995908
Related structures: EMD-4140

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 82-mer (preferred)
Entry contents:
76 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (82 distinct):
Protein DOM34 Chain: A1
Molecule details ›
Chain: A1
Length: 381 amino acids
Theoretical weight: 43.57 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P33309 (Residues: 1-381; Coverage: 99%)
Gene names: DOM34, N2016, YNL001W
Sequence domains:
40S ribosomal protein S0-A Chain: A2
Molecule details ›
Chain: A2
Length: 207 amino acids
Theoretical weight: 22.94 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32905 (Residues: 2-208; Coverage: 82%)
Gene names: NAB1, NAB1A, RPS0A, YGR214W, YST1
40S ribosomal protein S26-B Chain: a2
Molecule details ›
Chain: a2
Length: 98 amino acids
Theoretical weight: 11.15 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39939 (Residues: 2-99; Coverage: 82%)
Gene names: RPS26B, YER131W
40S ribosomal protein S1-A Chain: B2
Molecule details ›
Chain: B2
Length: 214 amino acids
Theoretical weight: 24.31 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P33442 (Residues: 20-233; Coverage: 84%)
Gene names: L9753.9, PLC1, RPS10A, RPS1A, YLR441C
40S ribosomal protein S27-A Chain: b2
Molecule details ›
Chain: b2
Length: 81 amino acids
Theoretical weight: 8.76 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P35997 (Residues: 2-82; Coverage: 99%)
Gene names: RPS27A, YKL156W
Sequence domains: Ribosomal protein S27
40S ribosomal protein S2 Chain: C2
Molecule details ›
Chain: C2
Length: 217 amino acids
Theoretical weight: 23.21 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P25443 (Residues: 34-250; Coverage: 85%)
Gene names: G2893, RPS2, RPS4, SUP38, SUP44, YGL123W
Sequence domains:
40S ribosomal protein S28-B Chain: c2
Molecule details ›
Chain: c2
Length: 63 amino acids
Theoretical weight: 7.12 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0C0X0 (Residues: 5-67; Coverage: 94%)
Gene names: L8479.5, RPS28B, RPS33B, YLR264W
Sequence domains: Ribosomal protein S28e
40S ribosomal protein S3 Chain: D2
Molecule details ›
Chain: D2
Length: 223 amino acids
Theoretical weight: 24.7 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05750 (Residues: 3-225; Coverage: 93%)
Gene names: N1653, RPS3, SUF14, YNL178W
Sequence domains:
40S ribosomal protein S29-A Chain: d2
Molecule details ›
Chain: d2
Length: 53 amino acids
Theoretical weight: 6.34 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P41057 (Residues: 4-56; Coverage: 95%)
Gene names: L8084.11, RPS29A, RPS36A, YLR388W, YS29A
40S ribosomal protein S4-A Chain: E2
Molecule details ›
Chain: E2
Length: 260 amino acids
Theoretical weight: 29.34 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX35 (Residues: 2-261; Coverage: 100%)
Gene names: J2186, RPS4A, RPS7B, YJR145C
Sequence domains:
40S ribosomal protein S30-A Chain: e2
Molecule details ›
Chain: e2
Length: 60 amino acids
Theoretical weight: 6.78 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX33 (Residues: 2-61; Coverage: 95%)
Gene names: L8003.23, RPS30A, YLR287BC, YLR287C-A
Sequence domains: Ribosomal protein S30
40S ribosomal protein S5 Chain: F2
Molecule details ›
Chain: F2
Length: 206 amino acids
Theoretical weight: 22.91 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P26783 (Residues: 20-225; Coverage: 92%)
Gene names: J2045, RPS2, RPS5, YJR123W
40S ribosomal protein S6-A Chain: G2
Molecule details ›
Chain: G2
Length: 226 amino acids
Theoretical weight: 25.9 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX37 (Residues: 1-226; Coverage: 96%)
Gene names: LPG18C, RPS101, RPS10B, RPS6A, YPL090C
Guanine nucleotide-binding protein subunit beta-like protein Chain: g2
Molecule details ›
Chain: g2
Length: 318 amino acids
Theoretical weight: 34.71 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38011 (Residues: 2-319; Coverage: 100%)
Gene names: ASC1, CPC2, YM9718.15C, YMR116C
Sequence domains: WD domain, G-beta repeat
40S ribosomal protein S7-A Chain: H2
Molecule details ›
Chain: H2
Length: 184 amino acids
Theoretical weight: 21 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P26786 (Residues: 4-187; Coverage: 97%)
Gene names: RPS30, RPS7A, YOR096W, YOR3177W
Sequence domains: Ribosomal protein S7e
40S ribosomal protein S8-A Chain: I2
Molecule details ›
Chain: I2
Length: 199 amino acids
Theoretical weight: 22.41 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX39 (Residues: 2-200; Coverage: 100%)
Gene names: RPS14A, RPS8A, YBL06.05, YBL0613, YBL072C
Sequence domains: Ribosomal protein S8e
40S ribosomal protein S9-A Chain: J2
Molecule details ›
Chain: J2
Length: 185 amino acids
Theoretical weight: 21.21 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: O13516 (Residues: 2-186; Coverage: 94%)
Gene names: RPS13A, RPS9A, YPL081W, YS11A
Sequence domains: S4 domain
40S ribosomal protein S10-A Chain: K2
Molecule details ›
Chain: K2
Length: 96 amino acids
Theoretical weight: 11.57 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q08745 (Residues: 1-96; Coverage: 91%)
Gene names: RPS10A, YOR293W
Sequence domains: Plectin/S10 domain
40S ribosomal protein S11-A Chain: L2
Molecule details ›
Chain: L2
Length: 155 amino acids
Theoretical weight: 17.65 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX47 (Residues: 2-156; Coverage: 99%)
Gene names: RPS11A, RPS18A, YD9813.03, YDR025W
Sequence domains:
40S ribosomal protein S12 Chain: M2
Molecule details ›
Chain: M2
Length: 124 amino acids
Theoretical weight: 13.33 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P48589 (Residues: 20-143; Coverage: 87%)
Gene names: RPS12, YOR369C
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
40S ribosomal protein S13 Chain: N2
Molecule details ›
Chain: N2
Length: 150 amino acids
Theoretical weight: 16.93 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05756 (Residues: 2-151; Coverage: 99%)
Gene names: D4252, RPS13, RPS13C, YD9609.18, YDR064W
Sequence domains: Ribosomal protein S15
40S ribosomal protein S14-A Chain: O2
Molecule details ›
Chain: O2
Length: 127 amino acids
Theoretical weight: 13.45 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P06367 (Residues: 11-137; Coverage: 93%)
Gene names: CRY1, RPL59, RPS14A, YCR031C, YCR31C
Sequence domains: Ribosomal protein S11
40S ribosomal protein S15 Chain: P2
Molecule details ›
Chain: P2
Length: 124 amino acids
Theoretical weight: 14.16 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q01855 (Residues: 8-131; Coverage: 87%)
Gene names: RPS15, RPS21, YOL040C
40S ribosomal protein S16-A Chain: Q2
Molecule details ›
Chain: Q2
Length: 141 amino acids
Theoretical weight: 15.66 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX51 (Residues: 3-143; Coverage: 99%)
Gene names: RP61R, RPS16A, YM9375.12, YMR143W
Sequence domains: Ribosomal protein S9/S16
40S ribosomal protein S17-B Chain: R2
Molecule details ›
Chain: R2
Length: 125 amino acids
Theoretical weight: 14.27 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P14127 (Residues: 2-126; Coverage: 92%)
Gene names: RP51B, RPS17B, YDR447C
40S ribosomal protein S18-A Chain: S2
Molecule details ›
Chain: S2
Length: 145 amino acids
Theoretical weight: 16.94 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX55 (Residues: 2-146; Coverage: 99%)
Gene names: D9461.35, RPS18A, YDR450W
Sequence domains: Ribosomal protein S13/S18
40S ribosomal protein S19-A Chain: T2
Molecule details ›
Chain: T2
Length: 143 amino acids
Theoretical weight: 15.81 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P07280 (Residues: 2-144; Coverage: 99%)
Gene names: RP55A, RPS16AA, RPS19A, YOL121C
40S ribosomal protein S20 Chain: U2
Molecule details ›
Chain: U2
Length: 107 amino acids
Theoretical weight: 12.19 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38701 (Residues: 15-121; Coverage: 88%)
Gene names: RPS20, URP2, YHL015W
Sequence domains: Ribosomal protein S10p/S20e
40S ribosomal protein S21-A Chain: V2
Molecule details ›
Chain: V2
Length: 87 amino acids
Theoretical weight: 9.76 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0C0V8 (Residues: 1-87; Coverage: 100%)
Gene names: RPS21A, RPS25, RPS25A, RPS26A, YKR057W
Sequence domains: Ribosomal protein S21e
40S ribosomal protein S22-A Chain: W2
Molecule details ›
Chain: W2
Length: 129 amino acids
Theoretical weight: 14.52 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0C0W1 (Residues: 2-130; Coverage: 99%)
Gene names: J0355, RPS22A, RPS24, RPS24A, YJL190C
Sequence domains: Ribosomal protein S8
40S ribosomal protein S23-A Chain: X2
Molecule details ›
Chain: X2
Length: 144 amino acids
Theoretical weight: 15.94 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX29 (Residues: 2-145; Coverage: 99%)
Gene names: G6178, RPS23A, RPS28A, YGR118W
Sequence domains: Ribosomal protein S12/S23
40S ribosomal protein S24-A Chain: Y2
Molecule details ›
Chain: Y2
Length: 134 amino acids
Theoretical weight: 15.23 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX31 (Residues: 2-135; Coverage: 99%)
Gene names: RPS24A, RPS24EA, YER074W
Sequence domains: Ribosomal protein S24e
40S ribosomal protein S25-A Chain: Z2
Molecule details ›
Chain: Z2
Length: 70 amino acids
Theoretical weight: 8 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q3E792 (Residues: 36-105; Coverage: 65%)
Gene names: RPS25A, RPS31A, YGR027C
40S ribosomal protein S31 Chain: f2
Molecule details ›
Chain: f2
Length: 71 amino acids
Theoretical weight: 8.1 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05759 (Residues: 82-152; Coverage: 47%)
Gene names: L9470.14, RPS31, RPS37, UBI3, YLR167W
Sequence domains: Ribosomal protein S27a
60S ribosomal protein L28 Chain: a5
Molecule details ›
Chain: a5
Length: 148 amino acids
Theoretical weight: 16.63 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P02406 (Residues: 2-149; Coverage: 99%)
Gene names: CYH2, RPL28, YGL103W
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
60S ribosomal protein L2-A Chain: A5
Molecule details ›
Chain: A5
Length: 252 amino acids
Theoretical weight: 27.22 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX45 (Residues: 2-253; Coverage: 99%)
Gene names: RPL2A, RPL5B, YFR031BC, YFR031C-A
Sequence domains: Ribosomal Proteins L2, C-terminal domain
60S ribosomal protein L29 Chain: b5
Molecule details ›
Chain: b5
Length: 58 amino acids
Theoretical weight: 6.56 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05747 (Residues: 2-59; Coverage: 98%)
Gene names: RPL29, YFR032BW, YFR032C-A, YL43
Sequence domains: Ribosomal L29e protein family
60S ribosomal protein L3 Chain: B5
Molecule details ›
Chain: B5
Length: 386 amino acids
Theoretical weight: 43.72 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P14126 (Residues: 2-387; Coverage: 100%)
Gene names: MAK8, RPL3, TCM1, YOR063W, YOR29-14
60S ribosomal protein L30 Chain: c5
Molecule details ›
Chain: c5
Length: 97 amino acids
Theoretical weight: 10.56 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P14120 (Residues: 9-105; Coverage: 92%)
Gene names: RPL30, RPL32, YGL030W
60S ribosomal protein L4-A Chain: C5
Molecule details ›
Chain: C5
Length: 361 amino acids
Theoretical weight: 39.03 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P10664 (Residues: 2-362; Coverage: 100%)
Gene names: RPL2, RPL2A, RPL4A, YBR0315, YBR031W
Sequence domains: Ribosomal protein L4/L1 family
60S ribosomal protein L5 Chain: D5
Molecule details ›
Chain: D5
Length: 296 amino acids
Theoretical weight: 33.63 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P26321 (Residues: 2-297; Coverage: 100%)
Gene names: LPI14W, RPL1, RPL1A, RPL5, YPL131W
Sequence domains:
60S ribosomal protein L31-A Chain: d5
Molecule details ›
Chain: d5
Length: 109 amino acids
Theoretical weight: 12.65 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0C2H8 (Residues: 4-112; Coverage: 97%)
Gene names: D2478, RPL31A, RPL34, RPL34A, YDL075W
60S ribosomal protein L32 Chain: e5
Molecule details ›
Chain: e5
Length: 127 amino acids
Theoretical weight: 14.48 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P38061 (Residues: 2-128; Coverage: 98%)
Gene names: RPL32, YBL0838, YBL092W
Sequence domains: Ribosomal protein L32
60S ribosomal protein L33-A Chain: f5
Molecule details ›
Chain: f5
Length: 106 amino acids
Theoretical weight: 12.05 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05744 (Residues: 2-107; Coverage: 99%)
Gene names: LPI4W, P2625, RPL33A, RPL37A, YPL143W
Sequence domains: Ribosomal protein L35Ae
60S ribosomal protein L7-A Chain: F5
Molecule details ›
Chain: F5
Length: 222 amino acids
Theoretical weight: 25.24 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05737 (Residues: 23-244; Coverage: 91%)
Gene names: RPL6A, RPL7A, RPL8A, YGL076C, YL8A
60S ribosomal protein L34-A Chain: g5
Molecule details ›
Chain: g5
Length: 112 amino acids
Theoretical weight: 12.61 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P87262 (Residues: 2-113; Coverage: 93%)
Gene names: RPL34A, YER056BC, YER056C-A
60S ribosomal protein L8-A Chain: G5
Molecule details ›
Chain: G5
Length: 233 amino acids
Theoretical weight: 25.81 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P17076 (Residues: 24-256; Coverage: 91%)
Gene names: MAK7, RPL4A, RPL8A, YHL033C
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
60S ribosomal protein L35-A Chain: h5
Molecule details ›
Chain: h5
Length: 119 amino acids
Theoretical weight: 13.81 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX84 (Residues: 2-120; Coverage: 99%)
Gene names: D1249, RPL35A, SOS1, YDL191W
60S ribosomal protein L9-A Chain: H5
Molecule details ›
Chain: H5
Length: 191 amino acids
Theoretical weight: 21.61 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05738 (Residues: 1-191; Coverage: 100%)
Gene names: RPL8A, RPL9, RPL9A, YGL147C
60S ribosomal protein L36-A Chain: i5
Molecule details ›
Chain: i5
Length: 99 amino acids
Theoretical weight: 11.02 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05745 (Residues: 2-100; Coverage: 99%)
Gene names: RPL36A, RPL39A, YM9646.06, YMR194W
60S ribosomal protein L10 Chain: I5
Molecule details ›
Chain: I5
Length: 220 amino acids
Theoretical weight: 25.28 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P41805 (Residues: 2-221; Coverage: 100%)
Gene names: GRC5, QSR1, RPL10, YLR075W
Sequence domains: Ribosomal protein L16p/L10e
60S ribosomal protein L11-A Chain: J5
Molecule details ›
Chain: J5
Length: 169 amino acids
Theoretical weight: 19.27 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0C0W9 (Residues: 6-174; Coverage: 97%)
Gene names: P8283.14, RP39A, RPL11A, RPL16A, YPR102C
Sequence domains:
60S ribosomal protein L37-A Chain: j5
Molecule details ›
Chain: j5
Length: 87 amino acids
Theoretical weight: 9.75 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P49166 (Residues: 2-88; Coverage: 99%)
Gene names: L9470.6, RPL35A, RPL37A, YLR185W
Sequence domains: Ribosomal protein L37e
60S ribosomal protein L38 Chain: k5
Molecule details ›
Chain: k5
Length: 77 amino acids
Theoretical weight: 8.71 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P49167 (Residues: 2-78; Coverage: 99%)
Gene names: L8543.2, RPL38, YLR325C
Sequence domains: Ribosomal L38e protein family
60S ribosomal protein L39 Chain: l5
Molecule details ›
Chain: l5
Length: 50 amino acids
Theoretical weight: 6.23 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P04650 (Residues: 2-51; Coverage: 98%)
Gene names: J0360, RPL39, RPL46, SPB2, YJL189W
60S ribosomal protein L13-A Chain: L5
Molecule details ›
Chain: L5
Length: 193 amino acids
Theoretical weight: 21.89 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q12690 (Residues: 2-194; Coverage: 97%)
Gene names: RPL13A, YDL082W
60S ribosomal protein L40-A Chain: m5
Molecule details ›
Chain: m5
Length: 52 amino acids
Theoretical weight: 6.03 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CH08 (Residues: 77-128; Coverage: 41%)
Gene names: RPL40A, UBI1, YIL148W
Sequence domains: Ribosomal L40e family
60S ribosomal protein L14-A Chain: M5
Molecule details ›
Chain: M5
Length: 136 amino acids
Theoretical weight: 14.98 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P36105 (Residues: 3-138; Coverage: 99%)
Gene names: RPL14A, YKL006W, YKL153
Sequence domains: Ribosomal protein L14
60S ribosomal protein L15-A Chain: N5
Molecule details ›
Chain: N5
Length: 203 amino acids
Theoretical weight: 24.35 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05748 (Residues: 2-204; Coverage: 100%)
Gene names: RPL10A, RPL13A, RPL15A, YL10A, YLR029C
Sequence domains: Ribosomal L15
60S ribosomal protein L42-B Chain: o5
Molecule details ›
Chain: o5
Length: 105 amino acids
Theoretical weight: 12.12 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX28 (Residues: 2-106; Coverage: 99%)
Gene names: MAK18, RPL41B, RPL42B, SCL41B, YHR141C
Sequence domains: Ribosomal protein L44
60S ribosomal protein L43-B Chain: p5
Molecule details ›
Chain: p5
Length: 91 amino acids
Theoretical weight: 9.98 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX26 (Residues: 2-92; Coverage: 99%)
Gene names: RPL43B, YJR094BW, YJR094W-A
Sequence domains: Ribosomal L37ae protein family
60S ribosomal protein L17-A Chain: P5
Molecule details ›
Chain: P5
Length: 183 amino acids
Theoretical weight: 20.46 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05740 (Residues: 2-184; Coverage: 100%)
Gene names: RPL17, RPL17A, RPL20A, YKL180W
60S ribosomal protein L18-A Chain: Q5
Molecule details ›
Chain: Q5
Length: 185 amino acids
Theoretical weight: 20.48 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX49 (Residues: 2-186; Coverage: 100%)
Gene names: RP28A, RPL18A, YOL120C
Sequence domains: Ribosomal protein 60S L18 and 50S L18e
60S ribosomal protein L20-A Chain: S5
Molecule details ›
Chain: S5
Length: 172 amino acids
Theoretical weight: 20.48 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX23 (Residues: 1-172; Coverage: 100%)
Gene names: RPL18A, RPL18A2, RPL20A, YM9408.04C, YMR242C
Sequence domains: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
60S ribosomal protein L22-A Chain: U5
Molecule details ›
Chain: U5
Length: 100 amino acids
Theoretical weight: 11.26 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05749 (Residues: 9-108; Coverage: 83%)
Gene names: L2168, RPL22A, YLR061W
60S ribosomal protein L23-A Chain: V5
Molecule details ›
Chain: V5
Length: 136 amino acids
Theoretical weight: 14.36 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX41 (Residues: 2-137; Coverage: 99%)
Gene names: RPL17A, RPL17AA, RPL23A, YBL0713, YBL087C
60S ribosomal protein L27-A Chain: Z5
Molecule details ›
Chain: Z5
Length: 135 amino acids
Theoretical weight: 15.44 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0C2H6 (Residues: 2-136; Coverage: 99%)
Gene names: RPL27, RPL27A, YHR010W
Sequence domains: Ribosomal L27e protein family
60S ribosomal protein L16-A Chain: K5
Molecule details ›
Chain: K5
Length: 197 amino acids
Theoretical weight: 22.03 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P26784 (Residues: 3-199; Coverage: 99%)
Gene names: RPL13, RPL16A, RPL21A, YIL133C
60S ribosomal protein L41-B Chain: n5
Molecule details ›
Chain: n5
Length: 25 amino acids
Theoretical weight: 3.35 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX87 (Residues: 1-25; Coverage: 100%)
Gene names: RPL41B, RPL47B, YDL133BC, YDL133C-A, YL41B
Sequence domains: Ribosomal protein L41
60S ribosomal protein L19-A Chain: R5
Molecule details ›
Chain: R5
Length: 188 amino acids
Theoretical weight: 21.63 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P0CX82 (Residues: 2-189; Coverage: 100%)
Gene names: RPL19A, RPL23A, YBR084BC, YBR084C-A, YL14A
Sequence domains: Ribosomal protein L19e
60S ribosomal protein L25 Chain: X5
Molecule details ›
Chain: X5
Length: 121 amino acids
Theoretical weight: 13.77 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P04456 (Residues: 22-142; Coverage: 85%)
Gene names: RPL25, YOL127W
60S ribosomal protein L26-A Chain: Y5
Molecule details ›
Chain: Y5
Length: 126 amino acids
Theoretical weight: 14.13 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P05743 (Residues: 2-127; Coverage: 99%)
Gene names: L8300.4, RPL26, RPL26A, RPL33A, YLR344W
Sequence domains:
60S ribosomal protein L21-A Chain: T5
Molecule details ›
Chain: T5
Length: 159 amino acids
Theoretical weight: 18.15 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q02753 (Residues: 2-160; Coverage: 99%)
Gene names: RPL21A, URP1, YBR1401, YBR191W
60S ribosomal protein L6-A Chain: E5
Molecule details ›
Chain: E5
Length: 175 amino acids
Theoretical weight: 19.87 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q02326 (Residues: 2-176; Coverage: 99%)
Gene names: RPL17A, RPL6A, YL16A, YML073C
60S ribosomal protein L24-A Chain: W5
Molecule details ›
Chain: W5
Length: 98 amino acids
Theoretical weight: 11.37 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P04449 (Residues: 1-98; Coverage: 63%)
Gene names: RP29, RPL24A, RPL30A, YGL031C
Sequence domains: Ribosomal protein L24e
Elongation factor 1 alpha-like protein Chain: A6
Molecule details ›
Chain: A6
Length: 611 amino acids
Theoretical weight: 68.81 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32769 (Residues: 1-611; Coverage: 100%)
Gene names: HBS1, YKR084C, YKR404
Sequence domains: Elongation factor Tu GTP binding domain
18S Ribosomal RNA Chain: 22
Molecule details ›
Chain: 22
Length: 1798 nucleotides
Theoretical weight: 579.13 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA
25S ribosomal RNA Chain: 14
Molecule details ›
Chain: 14
Length: 3396 nucleotides
Theoretical weight: 1097.49 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
5S ribosomal RNA Chain: 34
Molecule details ›
Chain: 34
Length: 121 nucleotides
Theoretical weight: 38.95 KDa
Sequence domains: 5S ribosomal RNA
5.8S ribosomal RNA Chain: 44
Molecule details ›
Chain: 44
Length: 158 nucleotides
Theoretical weight: 50.68 KDa
Sequence domains: 5.8S ribosomal RNA
nonstop mRNA Chain: X7
Molecule details ›
Chain: X7
Length: 20 nucleotides
Theoretical weight: 6.49 KDa
yeast Phe-tRNA-Phe Chain: A3
Molecule details ›
Chain: A3
Length: 76 nucleotides
Theoretical weight: 24.89 KDa
Sequence domains: tRNA

Ligands and Environments

4 bound ligands:
Ligand ZN 8 x ZN
Ligand MG 1058 x MG
Ligand GNP 1 x GNP
Ligand 5CR 1 x 5CR
11 modified residues:
Ligand 2MG 1 x 2MG
Ligand H2U 2 x H2U
Ligand M2G 1 x M2G
Ligand OMC 1 x OMC
Ligand OMG 1 x OMG
Ligand YYG 1 x YYG
Ligand PSU 2 x PSU
Ligand 5MC 2 x 5MC
Ligand 7MG 1 x 7MG
Ligand 5MU 1 x 5MU
Ligand 1MA 1 x 1MA

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-4140
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Targeting Eukaryotic mRNA Translation by Legionella pneumophila.
Belyi Y. (2020)
5 more