5m06

X-ray diffraction
2Å resolution

Crystal structure of Mycobacterium tuberculosis PknI kinase domain

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PknI Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 29.67 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WI69 (Residues: 1-256; Coverage: 44%)
Gene names: MTCY338.02c, Rv2914c, pknI
Sequence domains: Protein kinase domain
Structure domains: Phosphorylase Kinase; domain 1

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2221
Unit cell:
a: 60.004Å b: 69.541Å c: 257.417Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.239
Expression system: Escherichia coli BL21(DE3)