5lwh

X-ray diffraction
1.47Å resolution

CeuE (Y288F variant) a periplasmic protein from Campylobacter jejuni.

Released:
Source organism: Campylobacter jejuni
Entry authors: Wilde EJ, Blagova E, Hughes A, Raines DJ, Moroz OV, Turkenburg J, Duhme-Klair A-K, Wilson KS

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fe/B12 periplasmic-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 31.91 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0P8Q4 (Residues: 44-330; Coverage: 93%)
Gene names: Cj1355, ceuE
Sequence domains: Periplasmic binding protein
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P3221
Unit cell:
a: 65.521Å b: 65.521Å c: 145.654Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.13 0.128 0.162
Expression system: Escherichia coli BL21(DE3)