› 5lvw

X-ray diffraction
2.9Å resolution

XiaF (FADH2) from Streptomyces sp.

Released:
DOI: 10.2210/pdb5lvw/pdb
PDB entry 5lvw coloured by chain, front view.
PDB entry 5lvw coloured by chain, side view.
PDB entry 5lvw coloured by chain, top view.
Source organism: Streptomyces sp.
Primary publication:
Cryptic indole hydroxylation by a non-canonical terpenoid cyclase parallels bacterial xenobiotic detoxification.
Kugel S, Baunach M, Baer P, Ishida-Ito M, Sundaram S, Xu Z, Groll M, Hertweck C
Nat Commun 8 15804 (2017)
PMID: 28643772

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-125266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl-CoA dehydrogenase C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 413 amino acids
Theoretical weight: 44.24 KDa
Source organism: Streptomyces sp.
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: I7IIA9 (Residues: 3-399; Coverage: 100%)
Gene name: xiaF
Sequence domains: Acyl-CoA dehydrogenase, C-terminal domain
Structure domains: Butyryl-CoA Dehydrogenase, subunit A, domain 2

Ligands and Environments

3 bound ligands:
Ligand FAD 1 x FAD
Ligand IND 1 x IND
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P6222
Unit cell:
a: 166.6Å b: 166.6Å c: 99.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.174 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Enzymatic Cascade Reactions in Biosynthesis.
Walsh et al. (2019)
3 more

4 mentions without citation

Terpene synthases in disguise: enzymology, structure, and opportunities of non-canonical terpene synthases.
Rudolf et al. (2020)
3 more