› 5luh

X-ray diffraction
1.73Å resolution

AadA E87Q in complex with ATP, calcium and streptomycin

Released:
DOI: 10.2210/pdb5luh/pdb
PDB entry 5luh coloured by chain, front view.
PDB entry 5luh coloured by chain, side view.
PDB entry 5luh coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Primary publication:
Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica.
Stern AL, Van der Verren SE, Kanchugal P S, Näsvall J, Gutiérrez-de-Terán H, Selmer M
J Biol Chem 293 11481-11490 (2018)
PMID: 29871922

Function and Biology Details

Reaction catalysed: 
ATP + streptomycin = diphosphate + 3''-adenylylstreptomycin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187347 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside (3'') (9) adenylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 270 amino acids
Theoretical weight: 30.53 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZPX9 (Residues: 1-262; Coverage: 100%)
Gene names: STM1264, aadA
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand CA 4 x CA
Ligand ATP 2 x ATP
Ligand SRY 2 x SRY
Ligand EDO 1 x EDO
Ligand PEG 2 x PEG
Ligand CL 2 x CL
2 modified residues:
Ligand CSS 4 x CSS
Ligand CME 2 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P32
Unit cell:
a: 82.472Å b: 82.472Å c: 79.174Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 citation in other articles

In Silico Detection of Antimicrobial Resistance Integrons in Salmonella enterica Isolates from Countries of the Andean Community.
Torres-Elizalde et al. (2021)
18 more

2 mentions without citation

Structural mechanism of AadA, a dual-specificity aminoglycoside adenylyltransferase from Salmonella enterica.
Stern et al. (2018)
1 more