X-ray diffraction
2.1Å resolution

Crystal structure of human legumain (AEP) in complex with compound 11


Function and Biology Details

Reaction catalysed:
Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Legumain Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 29.94 KDa
Source organism: Homo sapiens
Expression system: Leishmania tarentolae
  • Canonical: Q99538 (Residues: 26-287; Coverage: 63%)
Gene names: LGMN, PRSC1
Sequence domains: Peptidase C13 family
Structure domains: Rossmann fold

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 43.52Å b: 75.04Å c: 172.97Å
α: 90° β: 90° γ: 90°
R R work R free
0.223 0.221 0.253
Expression system: Leishmania tarentolae