X-ray diffraction
2.41Å resolution

Crystal structure of phenylalanine ammonia-lyase from Anabaena variabilis (Y78F-C503S-C565S) bound to cinnamate


Function and Biology Details

Reaction catalysed:
L-phenylalanine = trans-cinnamate + ammonia
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Phenylalanine ammonia-lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 537 amino acids
Theoretical weight: 58.74 KDa
Source organism: Anabaena
Expression system: Escherichia coli BL21
  • Canonical: Q3M5Z3 (Residues: 25-563; Coverage: 95%)
Gene name: Ava_3988
Sequence domains: Aromatic amino acid lyase
Structure domains:

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P4322
Unit cell:
a: 78.077Å b: 78.077Å c: 354.492Å
α: 90° β: 90° γ: 90°
R R work R free
0.159 0.156 0.206
Expression system: Escherichia coli BL21