› 5ltc

X-ray diffraction
2.1Å resolution

Crystal structure of doubly spin labelled VcSiaP R125

Released:
DOI: 10.2210/pdb5ltc/pdb
PDB 5ltc coloured by chain and viewed from the front.
PDB 5ltc coloured by chain and viewed from the side.
PDB 5ltc coloured by chain and viewed from the top.
Source organism: Vibrio cholerae
Primary publication:
PELDOR Spectroscopy Reveals Two Defined States of a Sialic Acid TRAP Transporter SBP in Solution.
Glaenzer J, Peter MF, Thomas GH, Hagelueken G
Biophys. J. 112 109-120 (2017)
PMID: 28076802

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialic acid-binding periplasmic protein SiaP Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 37.33 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KR64 (Residues: 22-321; Coverage: 100%)
Gene names: VC_1779, siaP
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

No bound ligands
1 modified residue:
Ligand R1A 4 x R1A

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.3
Unit cell:
a: 72.27Å b: 78.1Å c: 116.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.262
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Selective Nutrient Transport in Bacteria: Multicomponent Transporter Systems Reign Supreme.
Davies et al. (2021)
1 more

1 mention without citation

PELDOR Spectroscopy Reveals Two Defined States of a Sialic Acid TRAP Transporter SBP in Solution.
Glaenzer et al. (2017)