PDBe 5ls6

X-ray diffraction
3.47Å resolution

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Histone-lysine N-methyltransferase EZH2 Chains: A, D, G, J
Molecule details ›
Chains: A, D, G, J
Length: 695 amino acids
Theoretical weight: 79.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q15910 (Residues: 1-385, 421-746; Coverage: 92%)
Gene names: EZH2, KMT6
Sequence domains:
Polycomb protein EED Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 367 amino acids
Theoretical weight: 42.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: O75530 (Residues: 77-441; Coverage: 83%)
Gene name: EED
Sequence domains: WD domain, G-beta repeat
Polycomb protein SUZ12 Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 129 amino acids
Theoretical weight: 15.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q15022 (Residues: 558-685; Coverage: 17%)
Gene names: CHET9, JJAZ1, KIAA0160, SUZ12
Protein Jumonji Chains: Q, R, S, T
Molecule details ›
Chains: Q, R, S, T
Length: 11 amino acids
Theoretical weight: 1.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q92833 (Residues: 110-120; Coverage: 1%)
Gene names: JARID2, JMJ

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 131.31Å b: 170.27Å c: 275.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.229 0.299
Expression systems:
  • Escherichia coli K-12
  • Not provided