X-ray diffraction
1.95Å resolution

Trehalose-6-phosphate synthase, GDP-glucose-dependent OtsA


Function and Biology Details

Reaction catalysed:
UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Alpha,alpha-trehalose-phosphate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 465 amino acids
Theoretical weight: 51.32 KDa
Source organism: Streptomyces venezuelae ATCC 10712
Expression system: Escherichia coli BL21(DE3)
  • Canonical: F2RH01 (Residues: 2-452; Coverage: 100%)
Gene name: SVEN_4043
Sequence domains: Glycosyltransferase family 20
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P21
Unit cell:
a: 41.43Å b: 168.35Å c: 133.92Å
α: 90° β: 97.19° γ: 90°
R R work R free
0.199 0.197 0.238
Expression system: Escherichia coli BL21(DE3)