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bMERB domains are bivalent Rab8 family effectors evolved by gene duplication.

Rai A, Oprisko A, Campos J, Fu Y, Friese T, Itzen A, Goody RS, Gazdag EM, Müller MP
Elife 5 (2016)
Related entries: 5szg, 5szh, 5szi, 5szj, 5szk

Cited: 35 times
EuropePMC logo PMID: 27552051

Abstract

In their active GTP-bound form, Rab proteins interact with proteins termed effector molecules. In this study, we have thoroughly characterized a Rab effector domain that is present in proteins of the Mical and EHBP families, both known to act in endosomal trafficking. Within our study, we show that these effectors display a preference for Rab8 family proteins (Rab8, 10, 13 and 15) and that some of the effector domains can bind two Rab proteins via separate binding sites. Structural analysis allowed us to explain the specificity towards Rab8 family members and the presence of two similar Rab binding sites that must have evolved via gene duplication. This study is the first to thoroughly characterize a Rab effector protein that contains two separate Rab binding sites within a single domain, allowing Micals and EHBPs to bind two Rabs simultaneously, thus suggesting previously unknown functions of these effector molecules in endosomal trafficking.

Reviews - 5lpn mentioned but not cited (2)

  1. Regulated methionine oxidation by monooxygenases. Manta B, Gladyshev VN. Free Radic Biol Med 109 141-155 (2017)
  2. Multivalency in Rab effector interactions. Rai A, Goody RS, Müller MP. Small GTPases 10 40-46 (2019)

Articles - 5lpn mentioned but not cited (7)

  1. Oxidation of F-actin controls the terminal steps of cytokinesis. Frémont S, Hammich H, Bai J, Wioland H, Klinkert K, Rocancourt M, Kikuti C, Stroebel D, Romet-Lemonne G, Pylypenko O, Houdusse A, Echard A. Nat Commun 8 14528 (2017)
  2. bMERB domains are bivalent Rab8 family effectors evolved by gene duplication. Rai A, Oprisko A, Campos J, Fu Y, Friese T, Itzen A, Goody RS, Gazdag EM, Müller MP. Elife 5 e18675 (2016)
  3. Inducible Exoc7/Exo70 knockout reveals a critical role of the exocyst in insulin-regulated GLUT4 exocytosis. Wang S, Crisman L, Miller J, Datta I, Gulbranson DR, Tian Y, Yin Q, Yu H, Shen J. J Biol Chem 294 19988-19996 (2019)
  4. The mechanism of activation of the actin binding protein EHBP1 by Rab8 family members. Rai A, Bleimling N, Vetter IR, Goody RS. Nat Commun 11 4187 (2020)
  5. Actin Cytoskeletal Reorganization Function of JRAB/MICAL-L2 Is Fine-tuned by Intramolecular Interaction between First LIM Zinc Finger and C-terminal Coiled-coil Domains. Miyake K, Sakane A, Tsuchiya Y, Sagawa I, Tomida Y, Kasahara J, Imoto I, Watanabe S, Higo D, Mizuguchi K, Sasaki T. Sci Rep 9 12794 (2019)
  6. Human MICAL1: Activation by the small GTPase Rab8 and small-angle X-ray scattering studies on the oligomerization state of MICAL1 and its complex with Rab8. Esposito A, Ventura V, Petoukhov MV, Rai A, Svergun DI, Vanoni MA. Protein Sci 28 150-166 (2019)
  7. Identifying cysteine residues susceptible to oxidation by photoactivatable atomic oxygen precursors using a proteome-wide analysis. Isor A, Chartier BV, Abo M, Currens ER, Weerapana E, McCulla RD. RSC Chem Biol 2 577-591 (2021)


Reviews citing this publication (5)

  1. Rab GTPases and their interacting protein partners: Structural insights into Rab functional diversity. Pylypenko O, Hammich H, Yu IM, Houdusse A. Small GTPases 9 22-48 (2018)
  2. Many roads to symmetry breaking: molecular mechanisms and theoretical models of yeast cell polarity. Goryachev AB, Leda M. Mol Biol Cell 28 370-380 (2017)
  3. Emerging roles of MICAL family proteins - from actin oxidation to membrane trafficking during cytokinesis. Frémont S, Romet-Lemonne G, Houdusse A, Echard A. J Cell Sci 130 1509-1517 (2017)
  4. MICAL-mediated oxidation of actin and its effects on cytoskeletal and cellular dynamics. Rajan S, Terman JR, Reisler E. Front Cell Dev Biol 11 1124202 (2023)
  5. Host Cell Signatures of the Envelopment Site within Beta-Herpes Virions. Mahmutefendić Lučin H, Blagojević Zagorac G, Marcelić M, Lučin P. Int J Mol Sci 23 9994 (2022)

Articles citing this publication (21)

  1. Rab10 regulates tubular endosome formation through KIF13A and KIF13B motors. Etoh K, Fukuda M. J Cell Sci 132 jcs226977 (2019)
  2. In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation. Gillingham AK, Bertram J, Begum F, Munro S. Elife 8 e45916 (2019)
  3. LRRK2 and Rab10 coordinate macropinocytosis to mediate immunological responses in phagocytes. Liu Z, Xu E, Zhao HT, Cole T, West AB. EMBO J 39 e104862 (2020)
  4. Mutations in LRRK2 linked to Parkinson disease sequester Rab8a to damaged lysosomes and regulate transferrin-mediated iron uptake in microglia. Mamais A, Kluss JH, Bonet-Ponce L, Landeck N, Langston RG, Smith N, Beilina A, Kaganovich A, Ghosh MC, Pellegrini L, Kumaran R, Papazoglou I, Heaton GR, Bandopadhyay R, Maio N, Kim C, LaVoie MJ, Gershlick DC, Cookson MR. PLoS Biol 19 e3001480 (2021)
  5. Multivalent Rab interactions determine tether-mediated membrane fusion. Lürick A, Gao J, Kuhlee A, Yavavli E, Langemeyer L, Perz A, Raunser S, Ungermann C. Mol Biol Cell 28 322-332 (2017)
  6. Comprehensive knockout analysis of the Rab family GTPases in epithelial cells. Homma Y, Kinoshita R, Kuchitsu Y, Wawro PS, Marubashi S, Oguchi ME, Ishida M, Fujita N, Fukuda M. J Cell Biol 218 2035-2050 (2019)
  7. Accurate MS-based Rab10 Phosphorylation Stoichiometry Determination as Readout for LRRK2 Activity in Parkinson's Disease. Karayel Ö, Tonelli F, Virreira Winter S, Geyer PE, Fan Y, Sammler EM, Alessi DR, Steger M, Mann M. Mol Cell Proteomics 19 1546-1560 (2020)
  8. PINK1-dependent phosphorylation of Serine111 within the SF3 motif of Rab GTPases impairs effector interactions and LRRK2-mediated phosphorylation at Threonine72. Vieweg S, Mulholland K, Bräuning B, Kachariya N, Lai YC, Toth R, Singh PK, Volpi I, Sattler M, Groll M, Itzen A, Muqit MMK. Biochem J 477 1651-1668 (2020)
  9. GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR ΔF508. Lucken-Ardjomande Häsler S, Vallis Y, Pasche M, McMahon HT. J Cell Biol 219 e201811014 (2020)
  10. F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis. Niu F, Sun K, Wei W, Yu C, Wei Z. Sci Adv 6 eabb1307 (2020)
  11. Facilitating identification of minimal protein binding domains by cross-linking mass spectrometry. Liu Q, Remmelzwaal S, Heck AJR, Akhmanova A, Liu F. Sci Rep 7 13453 (2017)
  12. Inferring joint sequence-structural determinants of protein functional specificity. Neuwald AF, Aravind L, Altschul SF. Elife 7 e29880 (2018)
  13. Salmonella effector SopD promotes plasma membrane scission by inhibiting Rab10. Boddy KC, Zhu H, D'Costa VM, Xu C, Beyrakhova K, Cygler M, Grinstein S, Coyaud E, Laurent EMN, St-Germain J, Raught B, Brumell JH. Nat Commun 12 4707 (2021)
  14. Genetic and structural studies of RABL3 reveal an essential role in lymphoid development and function. Zhong X, Su L, Yang Y, Nair-Gill E, Tang M, Anderton P, Li X, Wang J, Zhan X, Tomchick DR, Brautigam CA, Moresco EMY, Choi JH, Beutler B. Proc Natl Acad Sci U S A 117 8563-8572 (2020)
  15. Phosphoproteome Profiling of the Receptor Tyrosine Kinase MuSK Identifies Tyrosine Phosphorylation of Rab GTPases. Budayeva HG, Sengupta-Ghosh A, Phu L, Moffat JG, Ayalon G, Kirkpatrick DS. Mol Cell Proteomics 21 100221 (2022)
  16. MICALL2 as a substrate of ubiquitinase TRIM21 regulates tumorigenesis of colorectal cancer. Wen P, Wang H, Li Y, Sui X, Hou Z, Guo X, Xue W, Liu D, Wang Y, Gao J. Cell Commun Signal 20 170 (2022)
  17. Rep15 interacts with several Rab GTPases and has a distinct fold for a Rab effector. Rai A, Singh AK, Bleimling N, Posern G, Vetter IR, Goody RS. Nat Commun 13 4262 (2022)
  18. Case Reports Case Report: Identification of microduplication in the chromosomal 2p16.1p15 region in an infant suffering from pulmonary arterial hypertension. Wang X, Zhang Z, Zuo W, Wang D, Yang F, Liu Q, Xiao Y. Front Cardiovasc Med 10 1219480 (2023)
  19. EHBP1L1, an apicobasal polarity regulator, is critical for nuclear polarization during enucleation of erythroblasts. Wu J, Moriwaki K, Asuka T, Nakai R, Kanda S, Taniguchi M, Sugiyama T, Yoshimura SI, Kunii M, Nagasawa T, Hosen N, Miyoshi E, Harada A. Blood Adv 7 3382-3394 (2023)
  20. The Rab GTPase-binding protein EHBP1L1 and its interactors CD2AP/CIN85 negatively regulate the length of primary cilia via actin remodeling. Iwano T, Sobajima T, Takeda S, Harada A, Yoshimura SI. J Biol Chem 299 102985 (2023)
  21. mTORC2 suppresses cell death induced by hypo-osmotic stress by promoting sphingomyelin transport. Ono Y, Matsuzawa K, Ikenouchi J. J Cell Biol 221 e202106160 (2022)


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