5lpn

X-ray diffraction
2.8Å resolution

Structure of human Rab10 in complex with the bMERB domain of Mical-1

Released:

Function and Biology Details

Reaction catalysed:
[F-actin]-L-methionine + NADPH + O(2) + H(+) = [F-actin]-L-methionine-(R)-S-oxide + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-10 Chains: A, C
Molecule details ›
Chains: A, C
Length: 177 amino acids
Theoretical weight: 20.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61026 (Residues: 1-175; Coverage: 88%)
Gene name: RAB10
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
[F-actin]-monooxygenase MICAL1 Chain: B
Molecule details ›
Chain: B
Length: 153 amino acids
Theoretical weight: 17.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TDZ2 (Residues: 918-1067; Coverage: 14%)
Gene names: MICAL, MICAL1, NICAL
Sequence domains: Bivalent Mical/EHBP Rab binding domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 58.39Å b: 59Å c: 198.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.244 0.287
Expression system: Escherichia coli