5lpc Citations

Characterization of a Cyanobacterial Haloperoxidase and Evaluation of its Biocatalytic Halogenation Potential.

Chembiochem (2016)
Cited: 3 times
EuropePMC logo PMID: 27542168

Abstract

Vanadium-dependent haloperoxidases (VHPOs) are an exquisite class of halogenating enzymes found in fungi, lichen, algae and bacteria. We report the cloning, purification and characterization of a functional VHPO from the cyanobacterium Acaryochloris marina (AmVHPO), including its structure determination by X-ray crystallography. The AmVHPO features a unique set of disulfide bonds, which stabilize the dodecameric assembly of the protein. Easy access by high-yield recombinant expression as well as resistance towards organic solvents and temperature, together with a distinct halogenation reactivity, make this enzyme a promising starting point for the development of biocatalytic transformations.

Reviews citing this publication (1)

  1. Biocatalytic Oxidation Reactions: A Chemist's Perspective. Dong J, Fernández-Fueyo E, Hollmann F, Paul CE, Pesic M, Schmidt S, Wang Y, Younes S, Zhang W. Angew. Chem. Int. Ed. Engl. 57 9238-9261 (2018)

Articles citing this publication (2)

  1. Selective aerobic oxidation reactions using a combination of photocatalytic water oxidation and enzymatic oxyfunctionalisations. Zhang W, Fernández-Fueyo E, Ni Y, van Schie M, Gacs J, Renirie R, Wever R, Mutti FG, Rother D, Alcalde M, Hollmann F. Nat Catal 1 55-62 (2018)
  2. Halofunctionalization of alkenes by vanadium chloroperoxidase from Curvularia inaequalis. Dong JJ, Fernández-Fueyo E, Li J, Guo Z, Renirie R, Wever R, Hollmann F. Chem. Commun. (Camb.) 53 6207-6210 (2017)