5lop

X-ray diffraction
3.5Å resolution

Structure of the active form of /K. lactis/ Dcp1-Dcp2-Edc3 decapping complex bound to m7GDP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Nudix hydrolase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 32.63 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CIU1 (Residues: 1-275; Coverage: 34%)
Gene name: KLLA0_F23980g
Sequence domains:
KLLA0E01827p Chain: B
Molecule details ›
Chain: B
Length: 188 amino acids
Theoretical weight: 21.93 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CPV9 (Residues: 1-188; Coverage: 100%)
Gene name: KLLA0_E01827g
Sequence domains: Dcp1-like decapping family
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Enhancer of mRNA-decapping protein 3 Chain: C
Molecule details ›
Chain: C
Length: 66 amino acids
Theoretical weight: 7.33 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CX48 (Residues: 1-66; Coverage: 14%)
Gene name: KLLA0_A11308g

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6422
Unit cell:
a: 219.81Å b: 219.81Å c: 74.19Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.2 0.266
Expression system: Escherichia coli