X-ray diffraction
1.98Å resolution

Crystal structure of SCP2 thiolase from Leishmania mexicana. Complex of the C123A mutant with acetoacetyl-CoA.


Function and Biology Details

Reaction catalysed:
(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
3-ketoacyl-CoA thiolase-like protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 457 amino acids
Theoretical weight: 49.23 KDa
Source organism: Leishmania mexicana
Expression system: Escherichia coli BL21(DE3)
  • Canonical: E9AW84 (Residues: 1-441; Coverage: 100%)
Gene name: LMXM_23_0690
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

Cofactor: Ligand CAA 3 x CAA
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 74.141Å b: 89.804Å c: 123.716Å
α: 90° β: 104.76° γ: 90°
R R work R free
0.191 0.189 0.226
Expression system: Escherichia coli BL21(DE3)