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Crystal Structure of Saccharomyces cerevisiae ECM4, a Xi-Class Glutathione Transferase that Reacts with Glutathionyl-(hydro)quinones.

Schwartz M, Didierjean C, Hecker A, Girardet JM, Morel-Rouhier M, Gelhaye E, Favier F
PLoS One 11 e0164678 (2016)
Cited: 6 times
EuropePMC logo PMID: 27736955

Abstract

Glutathionyl-hydroquinone reductases (GHRs) belong to the recently characterized Xi-class of glutathione transferases (GSTXs) according to unique structural properties and are present in all but animal kingdoms. The GHR ScECM4 from the yeast Saccharomyces cerevisiae has been studied since 1997 when it was found to be potentially involved in cell-wall biosynthesis. Up to now and in spite of biological studies made on this enzyme, its physiological role remains challenging. The work here reports its crystallographic study. In addition to exhibiting the general GSTX structural features, ScECM4 shows extensions including a huge loop which contributes to the quaternary assembly. These structural extensions are probably specific to Saccharomycetaceae. Soaking of ScECM4 crystals with GS-menadione results in a structure where glutathione forms a mixed disulfide bond with the cysteine 46. Solution studies confirm that ScECM4 has reductase activity for GS-menadione in presence of glutathione. Moreover, the high resolution structures allowed us to propose new roles of conserved residues of the active site to assist the cysteine 46 during the catalytic act.

Articles - 5lkd mentioned but not cited (1)

  1. Crystal Structure of Saccharomyces cerevisiae ECM4, a Xi-Class Glutathione Transferase that Reacts with Glutathionyl-(hydro)quinones. Schwartz M, Didierjean C, Hecker A, Girardet JM, Morel-Rouhier M, Gelhaye E, Favier F. PLoS One 11 e0164678 (2016)


Reviews citing this publication (2)

  1. Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases. Sylvestre-Gonon E, Law SR, Schwartz M, Robe K, Keech O, Didierjean C, Dubos C, Rouhier N, Hecker A. Front Plant Sci 10 608 (2019)
  2. Unlocking Flavor Potential Using Microbial β-Glucosidases in Food Processing. Muradova M, Proskura A, Canon F, Aleksandrova I, Schwartz M, Heydel JM, Baranenko D, Nadtochii L, Neiers F. Foods 12 4484 (2023)

Articles citing this publication (3)

  1. Phylogenomic Insights into Distribution and Adaptation of Bdellovibrionota in Marine Waters. Li QM, Zhou YL, Wei ZF, Wang Y. Microorganisms 9 757 (2021)
  2. Structural Characterization of the Xi Class Glutathione Transferase From the Haloalkaliphilic Archaeon Natrialba magadii. Di Matteo A, Federici L, Masulli M, Carletti E, Santorelli D, Cassidy J, Paradisi F, Di Ilio C, Allocati N. Front Microbiol 10 9 (2019)
  3. Trametes versicolor glutathione transferase Xi 3, a dual Cys-GST with catalytic specificities of both Xi and Omega classes. Schwartz M, Perrot T, Deroy A, Roret T, Morel-Rouhier M, Mulliert G, Gelhaye E, Favier F, Didierjean C. FEBS Lett 592 3163-3172 (2018)


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