› 5lk5

X-ray diffraction
2.3Å resolution

Crystal structure of the globular domain of human calreticulin mutant D71K

Released:
DOI: 10.2210/pdb5lk5/pdb
PDB entry 5lk5 coloured by chain, front view.
PDB entry 5lk5 coloured by chain, side view.
PDB entry 5lk5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of parasite calreticulins provide insights into their flexibility and dual carbohydrate/peptide-binding properties.
Moreau C, Cioci G, Iannello M, Laffly E, Chouquet A, Ferreira A, Thielens NM, Gaboriaud C
IUCrJ 3 408-419 (2016)
PMID: 27840680

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151075 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calreticulin Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 265 amino acids
Theoretical weight: 30.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P27797 (Residues: 18-204, 303-368; Coverage: 63%)
Gene names: CALR, CRTC
Sequence domains: Calreticulin family
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand CA 10 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P42
Unit cell:
a: 196.976Å b: 196.976Å c: 67.806Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.247
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

5 review citations

Calnexin cycle - structural features of the ER chaperone system.
Kozlov et al. (2020)
4 more

5 mentions without citation

Effects of calreticulin mutations on cell transformation and immunity.
Desikan et al. (2023)
4 more