5lil

X-ray diffraction
3.35Å resolution

Structure of Aggregatibacter actinomycetemcomitans MacB bound to ATPyS (P21)

Released:
Primary publication:
Structure and mechanotransmission mechanism of the MacB ABC transporter superfamily.
Proc Natl Acad Sci U S A 114 12572-12577 (2017)
PMID: 29109272

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrolide export ATP-binding/permease protein MacB Chains: A, B
Molecule details ›
Chains: A, B
Length: 664 amino acids
Theoretical weight: 72.11 KDa
Source organism: Aggregatibacter actinomycetemcomitans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2EHL8 (Residues: 1-644; Coverage: 100%)
Gene name: macB
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 111.587Å b: 82.621Å c: 124.92Å
α: 90° β: 94.45° γ: 90°
R-values:
R R work R free
0.247 0.244 0.29
Expression system: Escherichia coli BL21(DE3)