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X-ray diffraction
3.35Å resolution

Structure of Aggregatibacter actinomycetemcomitans MacB bound to ATPyS (P21)

Released:
DOI: 10.2210/pdb5lil/pdb
PDB 5lil coloured by chain and viewed from the front.
PDB 5lil coloured by chain and viewed from the side.
PDB 5lil coloured by chain and viewed from the top.
Source organism: Aggregatibacter actinomycetemcomitans
Primary publication:
Structure and mechanotransmission mechanism of the MacB ABC transporter superfamily.
Crow A, Greene NP, Kaplan E, Koronakis V
Proc Natl Acad Sci U S A 114 12572-12577 (2017)
PMID: 29109272

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrolide export ATP-binding/permease protein MacB Chains: A, B
Molecule details ›
Chains: A, B
Length: 664 amino acids
Theoretical weight: 72.11 KDa
Source organism: Aggregatibacter actinomycetemcomitans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2EHL8 (Residues: 1-644; Coverage: 100%)
Gene name: macB
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P21
Unit cell:
a: 111.587Å b: 82.621Å c: 124.92Å
α: 90° β: 94.45° γ: 90°
R-values:
R R work R free
0.247 0.244 0.29
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Not Just Transporters: Alternative Functions of ABC Transporters in Bacillus subtilis and Listeria monocytogenes.
Rismondo et al. (2021)
6 more