› 5lid

X-ray diffraction
4.5Å resolution

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromopromazine

Released:
DOI: 10.2210/pdb5lid/pdb
PDB 5lid coloured by chain and viewed from the front.
PDB 5lid coloured by chain and viewed from the side.
PDB 5lid coloured by chain and viewed from the top.
Source organism: Dickeya chrysanthemi
Primary publication:
Allosteric binding site in a Cys-loop receptor ligand-binding domain unveiled in the crystal structure of ELIC in complex with chlorpromazine.
Nys M, Wijckmans E, Farinha A, Yoluk Ö, Andersson M, Brams M, Spurny R, Peigneur S, Tytgat J, Lindahl E, Ulens C
Proc Natl Acad Sci U S A 113 E6696-E6703 (2016)
PMID: 27791038

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cys-loop ligand-gated ion channel Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 307 amino acids
Theoretical weight: 35.38 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C7B7 (Residues: 11-163, 164-316; Coverage: 95%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain

Ligands and Environments

1 bound ligand:
Ligand 6XY 20 x 6XY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 105.72Å b: 267.91Å c: 111.33Å
α: 90° β: 106.7° γ: 90°
R-values:
R R work R free
0.215 0.212 0.257
Expression system: Escherichia coli

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Citations

2 review citations

Mutagenesis computer experiments in pentameric ligand-gated ion channels: the role of simulation tools with different resolution.
Crnjar et al. (2019)
1 more