5lid

X-ray diffraction
4.5Å resolution

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) in complex with bromopromazine

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cys-loop ligand-gated ion channel Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 307 amino acids
Theoretical weight: 35.38 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C7B7 (Residues: 11-163, 164-316; Coverage: 95%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21
Unit cell:
a: 105.72Å b: 267.91Å c: 111.33Å
α: 90° β: 106.7° γ: 90°
R-values:
R R work R free
0.215 0.212 0.257
Expression system: Escherichia coli