5lh0 › Citations

A multicrystal diffraction data-collection approach for studying structural dynamics with millisecond temporal resolution.

Schubert R, Kapis S, Gicquel Y, Bourenkov G, Schneider TR, Heymann M, Betzel C, Perbandt M
IUCrJ 3 393-401 (2016)
Related entries: 5lh1, 5lh3, 5lh5, 5lh6, 5lh7, 5lmh, 5ln0

Cited: 10 times
EuropePMC logo PMID: 27840678

Abstract

Many biochemical processes take place on timescales ranging from femto-seconds to seconds. Accordingly, any time-resolved experiment must be matched to the speed of the structural changes of interest. Therefore, the timescale of interest defines the requirements of the X-ray source, instrumentation and data-collection strategy. In this study, a minimalistic approach for in situ crystallization is presented that requires only a few microlitres of sample solution containing a few hundred crystals. It is demonstrated that complete diffraction data sets, merged from multiple crystals, can be recorded within only a few minutes of beamtime and allow high-resolution structural information of high quality to be obtained with a temporal resolution of 40 ms. Global and site-specific radiation damage can be avoided by limiting the maximal dose per crystal to 400 kGy. Moreover, analysis of the data collected at higher doses allows the time-resolved observation of site-specific radiation damage. Therefore, our approach is well suited to observe structural changes and possibly enzymatic reactions in the low-millisecond regime.

Articles - 5lh0 mentioned but not cited (1)

  1. A multicrystal diffraction data-collection approach for studying structural dynamics with millisecond temporal resolution. Schubert R, Kapis S, Gicquel Y, Bourenkov G, Schneider TR, Heymann M, Betzel C, Perbandt M. IUCrJ 3 393-401 (2016)


Articles citing this publication (9)

  1. Serial millisecond crystallography for routine room-temperature structure determination at synchrotrons. Weinert T, Olieric N, Cheng R, Brünle S, James D, Ozerov D, Gashi D, Vera L, Marsh M, Jaeger K, Dworkowski F, Panepucci E, Basu S, Skopintsev P, Doré AS, Geng T, Cooke RM, Liang M, Prota AE, Panneels V, Nogly P, Ermler U, Schertler G, Hennig M, Steinmetz MO, Wang M, Standfuss J. Nat Commun 8 542 (2017)
  2. Radiation damage and dose limits in serial synchrotron crystallography at cryo- and room temperatures. de la Mora E, Coquelle N, Bury CS, Rosenthal M, Holton JM, Carmichael I, Garman EF, Burghammer M, Colletier JP, Weik M. Proc Natl Acad Sci U S A 117 4142-4151 (2020)
  3. 3D-MiXD: 3D-printed X-ray-compatible microfluidic devices for rapid, low-consumption serial synchrotron crystallography data collection in flow. Monteiro DCF, von Stetten D, Stohrer C, Sans M, Pearson AR, Santoni G, van der Linden P, Trebbin M. IUCrJ 7 207-219 (2020)
  4. Sample manipulation and data assembly for robust microcrystal synchrotron crystallography. Guo G, Fuchs MR, Shi W, Skinner J, Berman E, Ogata CM, Hendrickson WA, McSweeney S, Liu Q. IUCrJ 5 238-246 (2018)
  5. Transforming X-ray detection with hybrid photon counting detectors. Förster A, Brandstetter S, Schulze-Briese C. Philos Trans A Math Phys Eng Sci 377 20180241 (2019)
  6. xia2.multiplex: a multi-crystal data-analysis pipeline. Gildea RJ, Beilsten-Edmands J, Axford D, Horrell S, Aller P, Sandy J, Sanchez-Weatherby J, Owen CD, Lukacik P, Strain-Damerell C, Owen RL, Walsh MA, Winter G. Acta Crystallogr D Struct Biol 78 752-769 (2022)
  7. Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography. Gicquel Y, Schubert R, Kapis S, Bourenkov G, Schneider T, Perbandt M, Betzel C, Chapman HN, Heymann M. J Vis Exp (2018)
  8. Determining biomolecular structures near room temperature using X-ray crystallography: concepts, methods and future optimization. Thorne RE. Acta Crystallogr D Struct Biol 79 78-94 (2023)
  9. Evaluation of the data-collection strategy for room-temperature micro-crystallography studied by serial synchrotron rotation crystallography combined with the humid air and glue-coating method. Hasegawa K, Baba S, Kawamura T, Yamamoto M, Kumasaka T. Acta Crystallogr D Struct Biol 77 300-312 (2021)


Quick links