5lev

X-ray diffraction
3.2Å resolution

Crystal structure of human UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1) (V264G mutant)

Released:
Source organism: Homo sapiens
Entry authors: Pike ACW, Dong YY, Chu A, Tessitore A, Goubin S, Dong L, Mukhopadhyay S, Mahajan P, Chalk R, Berridge G, Wang D, Kupinska K, Belaya K, Beeson D, Burgess-Brown N, Edwards AM, Arrowsmith CH, Bountra C, Carpenter EP, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 46.17 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9H3H5 (Residues: 1-408; Coverage: 100%)
Gene names: DPAGT1, DPAGT2
Sequence domains: Glycosyl transferase family 4

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P6522
Unit cell:
a: 103.25Å b: 103.25Å c: 239.15Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.234 0.24
Expression system: Spodoptera frugiperda