5ldg

X-ray diffraction
1.3Å resolution

Isopiperitenone reductase from Mentha piperita in complex with Isopiperitenone and NADP

Released:

Function and Biology Details

Reaction catalysed:
(+)-cis-isopulegone + NADP(+) = (-)-isopiperitenone + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(-)-isopiperitenone reductase Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 34.58 KDa
Source organism: Mentha x piperita
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6WAU1 (Residues: 1-314; Coverage: 100%)
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 60.56Å b: 65.49Å c: 95.109Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.12 0.12 0.146
Expression system: Escherichia coli