PDBe 5lba

X-ray diffraction
2.5Å resolution

Crystal structure of human RECQL5 helicase in complex with DSPL fragment(1-cyclohexyl-3-(oxolan-2-ylmethyl)urea, SGC - Diamond XChem I04-1 fragment screening.

Released:
Source organism: Homo sapiens
Entry authors: Newman JA, Aitkenhead H, Talon R, Savitsky P, Krojer T, von Delft F, Arrowsmith CH, Edwards AM, Bountra C, Gileadi O, Structural Genomics Consortium (SGC)

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase Q5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 445 amino acids
Theoretical weight: 49.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O94762 (Residues: 11-453; Coverage: 45%)
Gene names: RECQ5, RECQL5
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P1
Unit cell:
a: 63.926Å b: 85.261Å c: 105.915Å
α: 109.9° β: 90.02° γ: 97.01°
R-values:
R R work R free
0.24 0.237 0.303
Expression system: Escherichia coli BL21