X-ray diffraction
2.2Å resolution

The 2.2 A crystal structure of CYP109E1 from Bacillus megaterium bound with four corticosterone molecules


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Cytochrome P450 Chains: A, B
Molecule details ›
Chains: A, B
Length: 410 amino acids
Theoretical weight: 47.82 KDa
Source organism: Priestia megaterium DSM 319
Expression system: Escherichia coli BL21(DE3)
  • Canonical: D5DKI8 (Residues: 1-404; Coverage: 100%)
Gene name: BMD_3874
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P3221
Unit cell:
a: 120.418Å b: 120.418Å c: 140.803Å
α: 90° β: 90° γ: 120°
R R work R free
0.187 0.185 0.232
Expression system: Escherichia coli BL21(DE3)