› 5l87

X-ray diffraction
0.87Å resolution

Targeting the PEX14-PEX5 interaction by small molecules provides novel therapeutic routes to treat trypanosomiases.

Released:
DOI: 10.2210/pdb5l87/pdb
PDB entry 5l87 coloured by chain, front view.
PDB entry 5l87 coloured by chain, side view.
PDB entry 5l87 coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Inhibitors of PEX14 disrupt protein import into glycosomes and kill Trypanosoma parasites.
Dawidowski M, Emmanouilidis L, Kalel VC, Tripsianes K, Schorpp K, Hadian K, Kaiser M, Mäser P, Kolonko M, Tanghe S, Rodriguez A, Schliebs W, Erdmann R, Sattler M, Popowicz GM
Science 355 1416-1420 (2017)
PMID: 28360328

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisomal membrane protein PEX14 Chain: A
Molecule details ›
Chain: A
Length: 69 amino acids
Theoretical weight: 7.88 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IEW2 (Residues: 20-84; Coverage: 18%)
Gene name: PEX14
Sequence domains: Pex14 N-terminal domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

2 bound ligands:
Ligand 6RD 1 x 6RD
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 43.03Å b: 37.26Å c: 42.46Å
α: 90° β: 117.89° γ: 90°
R-values:
R R work R free
0.13 0.129 0.145
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The peroxisome: an update on mysteries 2.0.
Islinger et al. (2018)
7 more