› 5l6v

X-ray diffraction
2.67Å resolution

Crystal structure of E. coli ADP-glucose pyrophosphorylase (AGPase) in complex with a negative allosteric regulator adenosine monophosphate (AMP) - AGPase*AMP

Released:
DOI: 10.2210/pdb5l6v/pdb
PDB entry 5l6v coloured by chain, front view.
PDB entry 5l6v coloured by chain, side view.
PDB entry 5l6v coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural Basis of Glycogen Biosynthesis Regulation in Bacteria.
Cifuente JO, Comino N, Madariaga-Marcos J, López-Fernández S, García-Alija M, Agirre J, Albesa-Jové D, Guerin ME
Structure 24 1613-22 (2016)
PMID: 27545622

Function and Biology Details

Reaction catalysed: 
ATP + alpha-D-glucose 1-phosphate = diphosphate + ADP-glucose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Glucose-1-phosphate adenylyltransferase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P
Length: 431 amino acids
Theoretical weight: 48.76 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6V1 (Residues: 1-431; Coverage: 100%)
Gene names: JW3393, b3430, glgC
Sequence domains: Nucleotidyl transferase
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
2 bound ligands:
Ligand AMP 16 x AMP
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 166.883Å b: 148.193Å c: 180.164Å
α: 90° β: 115.48° γ: 90°
R-values:
R R work R free
0.24 0.239 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The Mycobacterium tuberculosis capsule: a cell structure with key implications in pathogenesis.
Kalscheuer et al. (2019)
1 more

1 mention without citation

Mechanistic insights into the allosteric regulation of bacterial ADP-glucose pyrophosphorylases.
Comino et al. (2017)