PDBe 5l3x

X-ray diffraction
2.75Å resolution

Crystal structure of negative elongation factor subcomplex NELF-AC

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Negative elongation factor A Chain: A
Molecule details ›
Chain: A
Length: 177 amino acids
Theoretical weight: 19.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H3P2 (Residues: 6-182; Coverage: 34%)
Gene names: NELFA, P/OKcl.15, WHSC2
Negative elongation factor C/D Chain: B
Molecule details ›
Chain: B
Length: 405 amino acids
Theoretical weight: 45.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IXH7 (Residues: 186-590; Coverage: 69%)
Gene names: HSPC130, NELFCD, NELFD, TH1, TH1L

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: I213
Unit cell:
a: 185.07Å b: 185.07Å c: 185.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.237 0.256
Expression system: Escherichia coli BL21(DE3)