5l3g

X-ray diffraction
3.1Å resolution

LSD1-CoREST1 in complex with polymyxin E (colistin)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Lysine-specific histone demethylase 1A Chain: A
Molecule details ›
Chain: A
Length: 730 amino acids
Theoretical weight: 81.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60341 (Residues: 123-852; Coverage: 86%)
Gene names: AOF2, KDM1, KDM1A, KIAA0601, LSD1
Sequence domains:
Structure domains:
REST corepressor 1 Chain: B
Molecule details ›
Chain: B
Length: 178 amino acids
Theoretical weight: 20.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UKL0 (Residues: 308-485; Coverage: 37%)
Gene names: KIAA0071, RCOR, RCOR1
Sequence domains: Myb-like DNA-binding domain
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
polymyxin E Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.17 KDa
Source organism: Paenibacillus polymyxa

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I222
Unit cell:
a: 119.864Å b: 179.49Å c: 236.985Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.197
Expression system: Escherichia coli BL21(DE3)