› 5l20

X-ray diffraction
1.45Å resolution

Crystal Structure of a Clostripain (BT_0727) from Bacteroides thetaiotaomicron ATCC 29148 in Complex with Peptide Inhibitor BTN-VLTK-AOMK

Released:
DOI: 10.2210/pdb5l20/pdb
PDB entry 5l20 coloured by chain, front view.
PDB entry 5l20 coloured by chain, side view.
PDB entry 5l20 coloured by chain, top view.
Source organisms:
Entry authors: Wolan DW, Roncase EJ

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-219518 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Clostripain-related protein Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.46 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8A9T8 (Residues: 30-172; Coverage: 36%)
Gene name: BT_0727
Sequence domains: Clostripain family
Clostripain-related protein Chain: B
Molecule details ›
Chain: B
Length: 229 amino acids
Theoretical weight: 25.97 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q8A9T8 (Residues: 173-393; Coverage: 56%)
Gene name: BT_0727
Sequence domains: Clostripain family
Peptide Inhibitor BTN-VLTK-AOMK Chain: C
Molecule details ›
Chain: C
Length: 5 amino acids
Theoretical weight: 684 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2221
Unit cell:
a: 79.207Å b: 102.885Å c: 84.186Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.15 0.179
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided

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