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human POGLUT1 in complex with Notch1 EGF12 and UDP

X-ray diffraction
1.5Å resolution
PDB entry 5l0r coloured by chain, front view.
PDB entry 5l0r coloured by chain, side view.
PDB entry 5l0r coloured by chain, top view.
Source organism: Homo sapiens
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Protein O-glucosyltransferase 1 and Neurogenic locus notch homolog protein 1
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Multimeric state: hetero dimer
Accessible surface area: 17869.33 Å2
Buried surface area: 3394.86 Å2
Dissociation area: 921.4 Å2
Dissociation energy (ΔGdiss): 7.05 kcal/mol
Dissociation entropy (TΔSdiss): 10.16 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-155482

Macromolecules

Protein O-glucosyltransferase 1
Chain: A
Length: 357 amino acids
Theoretical weight: 42.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NBL1 (Residues: 29-385; Coverage: 97%)
Gene names: C3orf9, CLP46, KTELC1, MDS010, MDSRP, POGLUT1, UNQ490/PRO1006
Pfam: Glycosyl transferase family 90
InterPro: Glycosyl transferase CAP10 domain
Protein O-glucosyltransferase 1 in PDB entry 5l0r, assembly 1, front view.
Protein O-glucosyltransferase 1 in PDB entry 5l0r, assembly 1, side view.
Protein O-glucosyltransferase 1 in PDB entry 5l0r, assembly 1, top view.
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Neurogenic locus notch homolog protein 1
Chain: B
Length: 42 amino acids
Theoretical weight: 4.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46531 (Residues: 452-491; Coverage: 2%)
Gene names: NOTCH1, TAN1
Pfam: EGF-like domain
InterPro:
CATH: Laminin
Neurogenic locus notch homolog protein 1 in PDB entry 5l0r, assembly 1, front view.
Neurogenic locus notch homolog protein 1 in PDB entry 5l0r, assembly 1, side view.
Neurogenic locus notch homolog protein 1 in PDB entry 5l0r, assembly 1, top view.
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