› 5kzm

X-ray diffraction
2.8Å resolution

Crystal structure of Tryptophan synthase alpha-beta chain complex from Francisella tularensis

Released:
DOI: 10.2210/pdb5kzm/pdb
PDB entry 5kzm coloured by chain, front view.
PDB entry 5kzm coloured by chain, side view.
PDB entry 5kzm coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Primary publication:
Conservation of the structure and function of bacterial tryptophan synthases.
Michalska K, Gale J, Joachimiak G, Chang C, Hatzos-Skintges C, Nocek B, Johnston SE, Bigelow L, Bajrami B, Jedrzejczak RP, Wellington S, Hung DT, Nag PP, Fisher SL, Endres M, Joachimiak A
IUCrJ 6 649-664 (2019)
PMID: 31316809

Function and Biology Details

Reaction catalysed: 
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177638 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tryptophan synthase alpha chain Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 29.56 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NE80 (Residues: 1-269; Coverage: 100%)
Gene names: FTT_1772c, trpA
Sequence domains: Tryptophan synthase alpha chain
Structure domains: Aldolase class I
Tryptophan synthase beta chain Chain: B
Molecule details ›
Chain: B
Length: 396 amino acids
Theoretical weight: 43.84 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NE79 (Residues: 1-396; Coverage: 100%)
Gene names: FTT_1773c, trpB
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand CA 2 x CA
2 modified residues:
Ligand MSE 14 x MSE
Ligand LLP 1 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 111.176Å b: 171.986Å c: 76.109Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Meta-QTL analysis in wheat: progress, challenges and opportunities.
Sharma et al. (2023)
1 more

2 mentions without citation

Catalytically impaired TrpA subunit of tryptophan synthase from Chlamydia trachomatis is an allosteric regulator of TrpB.
Michalska et al. (2021)
1 more