X-ray diffraction
1.47Å resolution

mouse POFUT1 in complex with O-glucosylated mouse Factor VII EGF1 and GDP

Source organism: Mus musculus

Function and Biology Details

Reactions catalysed:
GDP-beta-L-fucose + [protein]-(L-serine/L-threonine) = GDP + [protein]-3-O-(alpha-L-fucosyl)-(L-serine/L-threonine)
Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GDP-fucose protein O-fucosyltransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 40.46 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
  • Canonical: Q91ZW2 (Residues: 33-384; Coverage: 97%)
Gene name: Pofut1
Sequence domains: GDP-fucose protein O-fucosyltransferase
Structure domains:
Factor VII light chain Chain: B
Molecule details ›
Chain: B
Length: 40 amino acids
Theoretical weight: 4.42 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
  • Canonical: P70375 (Residues: 87-126; Coverage: 10%)
Gene names: Cf7, F7
Sequence domains: EGF-like domain
Structure domains: Laminin

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 51.965Å b: 67.138Å c: 109.357Å
α: 90° β: 90° γ: 90°
R R work R free
0.154 0.153 0.175
Expression systems:
  • Homo sapiens
  • Escherichia coli