PDBe 5kvy

X-ray diffraction
1.95Å resolution

CRYSTAL STRUCTURE OF THE TWO TANDEM RRM DOMAINS OF PUF60 BOUND TO A PORTION OF AN ADML PRE-MRNA 3' SPLICE SITE ANALOG

Released:
Entry authors: Hsiao H-H, Crichlow GV, Albright RA, Murphy JW, Lolis EJ, Braddock DT

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Poly(U)-binding-splicing factor PUF60 Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 23.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UHX1 (Residues: 118-316; Coverage: 36%)
Gene names: FIR, PUF60, ROBPI, SIAHBP1
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
DNA (30-MER) Chain: C
Molecule details ›
Chain: C
Length: 30 nucleotides
Theoretical weight: 8.86 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P31
Unit cell:
a: 62.438Å b: 62.438Å c: 83.332Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.196 0.232
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided