› 5kvv

X-ray diffraction
2.01Å resolution

Structure of Malate Dehydrogenase in complex with NADH from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb5kvv/pdb
PDB entry 5kvv coloured by chain, front view.
PDB entry 5kvv coloured by chain, side view.
PDB entry 5kvv coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Entry authors: Cheng YS, Sacchettini JC, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 35.15 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WK13 (Residues: 2-329; Coverage: 100%)
Gene names: MTV006.12, Rv1240, mdh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 2 x NAI
3 bound ligands:
Ligand GOL 2 x GOL
Ligand TRS 1 x TRS
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 52.206Å b: 78.169Å c: 154.402Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.218
Expression system: Escherichia coli

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