› 5kvi

X-ray diffraction
2Å resolution

Crystal structure of monomeric human apoptosis-inducing factor with E413A/R422A/R430A mutations

Released:
DOI: 10.2210/pdb5kvi/pdb
PDB 5kvi coloured by chain and viewed from the front.
PDB 5kvi coloured by chain and viewed from the side.
PDB 5kvi coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Defining NADH-Driven Allostery Regulating Apoptosis-Inducing Factor.
Brosey CA, Ho C, Long WZ, Singh S, Burnett K, Hura GL, Nix JC, Bowman GR, Ellenberger T, Tainer JA
Structure 24 2067-2079 (2016)
PMID: 27818101

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Apoptosis-inducing factor 1, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 59.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95831 (Residues: 78-613; Coverage: 87%)
  • Best match: O95831-4 (Residues: 78-322)
Gene names: AIF, AIFM1, PDCD8
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand FAD 1 x FAD
Ligand EPE 1 x EPE
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 64.623Å b: 81.802Å c: 108.323Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.194
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Evolving SAXS versatility: solution X-ray scattering for macromolecular architecture, functional landscapes, and integrative structural biology.
Brosey et al. (2019)
2 more

1 mention without citation

Whole exome sequencing identifies a novel variant in an apoptosis-inducing factor gene associated with X-linked recessive hearing loss in a Chinese family.
Wang et al. (2019)