› 5kva

X-ray diffraction
1.83Å resolution

Crystal Structure of sorghum caffeoyl-CoA O-methyltransferase (CCoAOMT)

Released:
DOI: 10.2210/pdb5kva/pdb
PDB 5kva coloured by chain and viewed from the front.
PDB 5kva coloured by chain and viewed from the side.
PDB 5kva coloured by chain and viewed from the top.
Source organism: Sorghum bicolor
Primary publication:
The Structure and Catalytic Mechanism of Sorghum bicolor Caffeoyl-CoA O-Methyltransferase.
Walker AM, Sattler SA, Regner M, Jones JP, Ralph J, Vermerris W, Sattler SE, Kang C
Plant Physiol 172 78-92 (2016)
PMID: 27457122

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
caffeoyl-CoA O-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 31.05 KDa
Source organism: Sorghum bicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: C5Z4W3 (Residues: 1-261; Coverage: 100%)
Gene name: SORBI_3010G052200
Sequence domains: O-methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

2 bound ligands:
Ligand SAM 2 x SAM
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 55.531Å b: 91.562Å c: 55.783Å
α: 90° β: 90.69° γ: 90°
R-values:
R R work R free
0.16 0.159 0.193
Expression system: Escherichia coli

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Citations

1 review citation

Significance and Transformation of 3-Alkyl-2-Methoxypyrazines Through Grapes to Wine: Olfactory Properties, Metabolism, Biochemical Regulation, and the HP-MP Cycle.
Zhao et al. (2019)
17 other articles