› 5kof

X-ray diffraction
2.4Å resolution

Crosslinked Crystal Structure of Type II Fatty Acid Synthase Ketosynthase, FabB, and Acyl Carrier Protein, AcpP

Released:
DOI: 10.2210/pdb5kof/pdb
PDB entry 5kof coloured by chain, front view.
PDB entry 5kof coloured by chain, side view.
PDB entry 5kof coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Molecular basis for interactions between an acyl carrier protein and a ketosynthase.
Milligan JC, Lee DJ, Jackson DR, Schaub AJ, Beld J, Barajas JF, Hale JJ, Luo R, Burkart MD, Tsai SC
Nat Chem Biol 15 669-671 (2019)
PMID: 31209348

Function and Biology Details

Reaction catalysed: 
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141281 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
3-oxoacyl-[acyl-carrier-protein] synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 407 amino acids
Theoretical weight: 42.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A953 (Residues: 1-406; Coverage: 100%)
Gene names: JW2320, b2323, fabB, fabC
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1
Acyl carrier protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 78 amino acids
Theoretical weight: 8.65 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A6A8 (Residues: 1-78; Coverage: 100%)
Gene names: JW1080, acpP, b1094
Sequence domains: Phosphopantetheine attachment site

Ligands and Environments

1 bound ligand:
Ligand 6W5 2 x 6W5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 59.33Å b: 103.92Å c: 83.4Å
α: 90° β: 110.61° γ: 90°
R-values:
R R work R free
0.21 0.21 0.229
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

4 review citations

Probing the structure and function of acyl carrier proteins to unlock the strategic redesign of type II polyketide biosynthetic pathways.
Sulpizio et al. (2021)
3 more

9 mentions without citation

A Review of Fatty Acid Biosynthesis Enzyme Inhibitors as Promising Antimicrobial Drugs.
Bibens et al. (2023)
8 more