5knn

X-ray diffraction
2.68Å resolution

Evolutionary gain of alanine mischarging to non-cognate tRNAs with a G4:U69 base pair

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine--tRNA ligase, cytoplasmic Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 450 amino acids
Theoretical weight: 50.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4
UniProt:
  • Canonical: P49588 (Residues: 4-453; Coverage: 47%)
Gene names: AARS, AARS1
Structure domains: Bira Bifunctional Protein; Domain 2

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P1
Unit cell:
a: 52.167Å b: 98.26Å c: 201.385Å
α: 90.07° β: 89.95° γ: 90.11°
R-values:
R R work R free
0.218 0.216 0.254
Expression system: Escherichia coli-Pichia pastoris shuttle vector pPpARG4