5kl1

X-ray diffraction
3.7Å resolution

Crystal structure of the Pumilio-Nos-hunchback RNA complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
Maternal protein pumilio Chain: A
Molecule details ›
Chain: A
Length: 337 amino acids
Theoretical weight: 38.73 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P25822 (Residues: 1091-1426; Coverage: 22%)
Gene names: CG9755, pum
Sequence domains: Pumilio-family RNA binding repeat
Structure domains: Leucine-rich Repeat Variant
Protein nanos Chain: B
Molecule details ›
Chain: B
Length: 114 amino acids
Theoretical weight: 12.96 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P25724 (Residues: 289-401; Coverage: 28%)
Gene names: CG5637, nos
Sequence domains: Nanos RNA binding domain
Structure domains: Nanos, RNA-binding domain
RNA (5'-R(*AP*AP*AP*UP*UP*GP*UP*AP*CP*AP*UP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 5.09 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6522
Unit cell:
a: 137.007Å b: 137.007Å c: 221.401Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.268 0.264 0.3
Expression system: Escherichia coli