X-ray diffraction
3.51Å resolution

KSR2:MEK1 Complex Bound to the Small Molecule APS-2-79


Function and Biology Details

Reaction catalysed:
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kinase suppressor of Ras 2 Chain: B
Molecule details ›
Chain: B
Length: 319 amino acids
Theoretical weight: 36.77 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: Q6VAB6 (Residues: 634-950; Coverage: 33%)
Gene name: KSR2
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:
Dual specificity mitogen-activated protein kinase kinase 1 Chain: C
Molecule details ›
Chain: C
Length: 395 amino acids
Theoretical weight: 43.64 KDa
Source organism: Oryctolagus cuniculus
Expression system: Spodoptera frugiperda
  • Canonical: P29678 (Residues: 1-393; Coverage: 100%)
Gene names: MAP2K1, MEK1, PRKMK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P6122
Unit cell:
a: 139.68Å b: 139.68Å c: 221.73Å
α: 90° β: 90° γ: 120°
R R work R free
0.252 0.25 0.287
Expression system: Spodoptera frugiperda